Year: 2015 | Month: April | Volume 5 | Issue 1
Association of Semen Attributes and Seminal Plasma Proteins of Buffalo Bulls
Abstract:
This study was conducted to explicate the association of semen attributes with seminal plasma proteins of buffalo bulls. Total 108 ejaculates were collected from six sexually mature adult Bhadawari buffalo bulls aged 2-4 years in three seasons (rainy, winter and summer) of a year by using artificial vagina. Immediately after collection, semen samples were divided into two aliquots. One aliquots of neat semen were evaluated for ejaculate volume (EV), sperm concentration (SC), mass motility (MM), progressive motility (PM), percent live-dead (LD) count, percent Hypo-osmotic swelling test (HOST), and percent acrosomal integrity (AI). The other semen aliquots were centrifuged for harvesting the seminal plasma. SDS-PAGE was performed for separation of seminal plasma proteins and gel images were analysed to determine molecular weights, IOD of protein bands and relative protein fractions (protein %) using the Gel doc system. The correlation results revealed positive correlation of SC with 70 and 72 kDa proteins while negative correlation with 86 kDa protein. The PM showed positive correlation with 24.5, 70 and 72 kDa proteins and negative correlation with 84 and 86 kDa proteins and AI showed positive correlation with 18.5, 24.5, 44.5, 70, and 72 kDa and negative correlation with 20 and 84 kDa proteins. The results of correlation among seminal plasma proteins showed positive correlation of 24.5 kDa with 35, 44.5, 70 and 72 kDa and negative correlation with 86 kDa proteins. The 70 and 72 kDa proteins showed positive correlation with 18.5 and 24.5 kDa and negative correlation with 20, 84 and 86 kDa proteins. The 84 kDa proteins showed negative association with 24.5, 70 and 72 kDa proteins while 86 kDa proteins showed negative association with 24.5, 35, 36.5, 70 and 72 kDa proteins. In conclusion, though significant correlations among seminal plasma proteins and semen characteristics were detected, yet it is noteworthy that correlation does not mean cause. Therefore, more refined studies that allow higher-resolution separation of seminal plasma proteins and more detailed characterization of those proteins, as well as investigation of their physiological role, will further advance knowledge in this area.
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